SD CFAR MCI Service Request Form

Service Request Form

SD CFAR Molecular and Cellular Immunology Core

Please fill out the following form to submit a service request. Email cfar@ucsd.edu with any questions.

Contact Information

Requestor's First Name

Requestor's Last Name

Email

Institution administering this study

Are you the Principal Investigator for this project?

Yes
No

Principal Investigator Information

First Name

Last Name

Email

Phone Number (Please provide best phone number for us to contact you with time-sensitive questions)

Administrator Information (Person to contact regarding payment for services)

Administrator First and Last Name

Administrator Email

If you have a PO number or know the string number/payment information for internal recharge, please enter it here.

Project Details

Is this project related to HIV/AIDS?

Yes
No

Is this service for a funded project?

Yes
No

Is the project NIH-sponsored?

Yes
No

Funding source for non-NIH project. Provide grant number if available.

NIH Grant Number

NIH Grant Title

Link to project on NIH reporter
Not required, but this is helpful in ensuring the project is recorded correctly for NIH-reporting purposes. https://reporter.nih.gov/

Project Name (if different than grant title)

Service Request

Please select the MCI Core Unit(s) from which you are requesting service(s).

Genomics Unit
Flow Cytometry Unit
Single-Cell -Omics Unit
I'm not sure which services I need / which unit to select

Flow Cytometry Services Requested. More than one service can be selected.

Select the service you are requesting from below. Read the instrumentation section on the SD CFAR website to determine which machine to schedule for your experiment.

Analysis - BD CANTO (RUO GREEN)
Analysis - BD CANTO (RUO ORANGE)
Sort - ARIA II (Non-Biohazard sort)
Sort - ARIA II (Biohazard sort with bioBUBBLE)
Sort - NanoCellect WOLF (gentle cell sorting)
Training (please specify)
Other (please describe)

Experiment Type - Sorting

Immuno cell sorting
GFP sorting
Autoclone 96-well
Other (please describe)

Number of Samples/Tubes to be Sorted

Number of Cells per Sample to be Sorted
Specify Total Amount of cells in each Sample (NOT the # of cells to collected/sorted)
NOTE: Concentrate each sample ~20 million cells in 1.0 mL volume. If less than 20 million, resuspend in 0.5mL volume (bring extra suspension media/buffer).

FIRST Choice of Date & Time for Acquistion/Sorting * Type DATE and TIME (eg. Tues, 5/2/12 @ 1:30pm). Refer to our calendar for available time slots.

SECOND Choice of Date & Time for Acquistion/Sorting * Type DATE and TIME (eg. Tues, 5/2/12 @ 1:30pm). Refer to our calendar for available time slots.

Single-Cell -Omics Services Requested. More than one service can be selected.

Select the service you are requesting from the list below.

Sequencing

Illumina NovaSeq
Illumina NextSeq
ONT Minion
ONT P2

Data Analysis

adaptive sampling (ONT)
post-run basecalling (ONT)
CellRanger processing (10x Genomics)

Please provide a brief description of your study and services needed. Include any relevant information that will be helpful in providing the requested service(s).

Animal Subjects. Are the samples or data derived from animal subjects? By selecting "Yes" you are confirming that you have the appropriate approvals in place (i.e., IACUC approval).

Yes
No

Human Subjects. Are the samples or data derived from human subjects? By selecting "Yes" you are confirming that you have the appropriate approvals in place (i.e., IRB approval).

Yes
No

How did you hear about us? Please help us understand how you learned about our services so we can target out outreach and best support investigators.

Acknowledgment. I agree to acknowledge the CFAR grant on all publications, abstracts, grant proposals and presentations resulting from work supported by these services. Please refer to Cite the CFAR for details. This will also be emailed to you.

Yes